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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
31.25-2000 pg/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Serum, plasma, tissue homogenates and other biological fluids
- 灵敏度:
11.5 pg/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 人T细胞分化抗原CD6(CD6)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human CD6(T-cell differentiation antigen CD6) ELISA Kit |
| 别名 | T12; TP120; Soluble CD6 |
| 货号 | ELK9910 |
| 反应种属 | Human |
| P30203 | P30203 |
| 检测类型 | Sandwich |
| 灵敏度 | 11.5 pg/mL |
| 标准品 | 2000 pg/mL |
| 检测范围 | 31.25-2000 pg/mL |
| 样本类型 | Serum, plasma, tissue homogenates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD6 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction;Infection immunity; |

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文献和实验CD6 分子 常用单克隆抗体或代号:T12,T411 主要表达细胞:TsuL,Bsub,Thy[T] 分子质量(kDa)和结构:gp100(清除剂受体) 功 能:配体CDl66,T细胞活化,胸腺细胞与基质细胞相互作用 Adhesion molecule mediating the binding of developing thymocytes with thymic epithelial cells May be involved
80年代初以来,由于单克隆抗体,分子克隆、基因转染细胞系等技术在白细胞分化抗原研究中得到广泛深入的应用,有关白细胞分化抗原的研究和应用进展相当迅速。在世界卫生组织(WHO)和国际免疫学会联合会(IUIS)的组织下,自1982年至1993年已先后举行了五次有关人类白细胞分化抗原的国际协作组 会议 (International workshop on human leukocyte differentiation antigens),并应用以单克隆抗体鉴定为主的聚类
Reagents : PBS (pH 7.3) Coating Solution: 0.1 M Sodium Carbonate (pH9.6) with 0.02% Sodium Azide Wash Solution: 0.9% NaCl-0.05% Tween 20 (NaCl/T) PBS-TA: PBS-Tween 20 (0.05%) - sodium azide (0.02
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