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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.78-50 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Mouse
- 样本:
Tissue homogenates, cell lysates and other biological fluids
- 灵敏度:
0.19 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 小鼠微管关联蛋白1轻链3α(MAP1LC3a)酶联免疫吸附检测试剂盒 |
| 英文名称 | Mouse MAP1LC3a(Microtubule Associated Protein 1 Light Chain 3 Alpha) ELISA Kit |
| 别名 | MAP1ALC3; MAP1BLC3; LC3; LC3A; ATG8E; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1 |
| 货号 | ELK9909 |
| 反应种属 | Mouse |
| Q91VR7 | Q91VR7 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.19 ng/mL |
| 标准品 | 50 ng/mL |
| 检测范围 | 0.78-50 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MAP1LC3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MAP1LC3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MAP1LC3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MAP1LC3a in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Metabolic pathway; |

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文献和实验Screening for Inhibitors of Microtubule-Associated Motor Proteins
The mitotic spindle is an important target for cancer chemotherapy. The main protein target for drugs in clinical use is tubulin, the building block of microtubules. In recent years, other proteins of the mitotic spindle have been identified
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Variations on the Single-Molecule Assay for Microtubule-Associated Proteins and Kinesins
The direct observation of single kinesins and microtubule-associated proteins (MAPs) has become a core tool for cytoskeleton research. We outline several variations to the core experiment that allow the researcher to explore structural
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