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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Mouse
- 样本:
Tissue homogenates, cell lysates and other biological fluids
- 灵敏度:
0.049 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 小鼠多聚ADP核糖聚合酶(PARP)酶联免疫吸附检测试剂盒 |
| 英文名称 | Mouse PARP(Poly ADP Ribose Polymerase) ELISA Kit |
| 别名 | PARP1; ADPRT; ADPRT1; PPOL; pADPRT-1; ADP-ribosyltransferase diphtheria toxin-like 1; NAD(+) ADP-ribosyltransferase 1 |
| 货号 | ELK9818 |
| 反应种属 | Mouse |
| P11103 | P11103 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.049 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PARP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PARP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PARP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PARP in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Enzyme & Kinase; |

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文献和实验的Caspase-3及对底物多聚(ADP-核糖)聚合酶[poly(ADP-ribose)polymerase,PARP]等的裂解。方法: 收集细胞→PBS洗涤→抽提细胞裂解液→蛋白定量→SDS-PAGE电泳→硝酸纤维素膜或PVDF膜转移→5%脱脂奶粉封闭,室温1.5~2h或4°C过夜→Caspase-3多抗或单抗室温反应1~2h或4°C过夜→TBS-T(含0.05% Tween 20的TBS)洗3次,5~10min/次→HRP-标记的羊抗鼠IgG或AP标记的羊抗鼠IgG室温反应1~2h→ TBS-T洗3次
的Caspase-3及对底物多聚(ADP-核糖)聚合酶[poly(ADP-ribose)polymerase,PARP]等的裂解。方法:收集细胞→PBS洗涤→抽提细胞裂解液→蛋白定量→SDS-PAGE电泳→硝酸纤维素膜或PVDF膜转移→5%脱脂奶粉封闭,室温1.5~2h或4°C过夜→Caspase-3多抗或单抗室温反应1~2h或4°C过夜→TBS-T(含0.05% Tween 20的TBS)洗3次,5~10min/次→HRP-标记的羊抗鼠IgG或AP标记的羊抗鼠IgG室温反应1~2h→ TBS-T洗3次
三句话读懂一篇 CNS:经常喝咖啡对心脏健康有何影响?| 可让谷物增产约 20%,中国团队发现关键基因
Communications 5. PNAS:抑制 PARP1 延长寿命新机制 研究表明,聚 ( ADP 核糖) 聚合酶-1 ( PARP1)在长寿中发挥重要作用。 2023 年 3 月 22 日,复旦大学刘铁民及孔星星共同通讯在 PNAS 杂志发表研究论文 Muscle PARP1 inhibition extends lifespan through AMPKα PARylation and activation in Drosophila。 该研究发现肌肉中 PARP1 的敲低能够延长果蝇的寿命
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