大鼠cAMP反应元件结合蛋白(CREB)酶联免疫吸附检测试剂
盒【Rat CREB(Cyclic AMP Response Element Binding Protein) ELISA Kit】- ￥1820 - 2600
- ELK Biotechnology
- 0.32-20 ng/mL
- 0.132 ng/mL
- 武汉
- ELK9706
- 2026年04月25日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
0.32-20 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Rat
- 样本：
Tissue homogenates and other biological fluids.
- 灵敏度：
0.132 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1820.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2600.0 |
| 中文名称 | 大鼠cAMP反应元件结合蛋白(CREB)酶联免疫吸附检测试剂盒 |
| 英文名称 | Rat CREB(Cyclic AMP Response Element Binding Protein) ELISA Kit |
| 别名 | CREB; active transcription factor CREB; cAMP responsive element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB-1; cyclic AMP-responsive element-binding protein 1; MGC9284; transactivator protein |
| 货号 | ELK9706 |
| 反应种属 | Rat |
| P15337 | P15337 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.132 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | Tissue homogenates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CREB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CREB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CREB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CREB in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Epigenetics and Nuclear Signaling; Immunology; Neuroscience |
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文献和实验The ability of prototypical second messenger cyclic AMP (cAMP) to positively control transcription of the somatostatin gene was pivotal to the original identification of the transcription factor cAMP response element-binding protein
-L1 fibroblasts or differentiating adipocytes. Reporter gene assays showed that there are two promoter regions in the 5′-flanking region in the mPDE3B gene: a distal region located approx 4 kb upstream of the translation initiation site that contains cAMP-response
., Fontana, M.F., Hyodo, M., Joncker, N.T., Ishii, K.J., Akira, S., Colonna, M., Chen, Z.J., Fitzgerald, K.A., Hayakawa, Y., and Vance, R.E. 2009. A host type I interferon response is induced by cytosolic sensing of the bacterial second messenger cyclic‐di
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