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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.79-50 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Rat
- 样本:
serum, plasma, tissue homogenates and other biological fluids
- 灵敏度:
0.3 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1680.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2400.0 |
| 中文名称 | 大鼠信号素4D(SEMA4D)酶联免疫吸附检测试剂盒 |
| 英文名称 | Rat SEMA4D(Semaphorin 4D) ELISA Kit |
| 别名 | CD100; A8; BB18; GR3; M-Sema G; M-Sema-G; SEMAJ; Coll-4; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 4D |
| 货号 | ELK9701 |
| 反应种属 | Rat |
| 检测类型 | Sandwich |
| 灵敏度 | 0.3 ng/mL |
| 标准品 | 50 ng/mL |
| 检测范围 | 0.79-50 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SEMA4D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SEMA4D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SEMA4D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SEMA4D in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule; |

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文献和实验相关实验
,因此,必须应用酶联免疫吸附试验(ELISA),间接血凝试验(PHA),放射免疫测定(RIA)、直接和间接荧光抗体技术(DFA、IFA)以及免疫酶斑点试验等进行筛选和克隆化。首先初筛出能分泌与预定抗原起反应的McAb杂交瘤细胞,再进一步从中筛选出有预定特异性的杂交瘤细胞,然后选出可供实际应用,具有能稳定生长和有功能特性的细胞克隆。为了防止无关克隆的过度生长,对阳性孔需进一步克隆化。常用的克隆方法有:有限稀释法(limiting dilution,LD)、软琼脂法、直接挑取法及荧光激活细胞分离仪(FACS
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大鼠信号素4D(SEMA4D)酶联免疫吸附检测试剂盒【Rat SEMA4D(Semaphorin 4D) ELISA Kit】
¥1680 - 2400





