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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.32-20 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Horse
- 样本:
Serum, plasma, tissue homogenates and other biological fluids
- 灵敏度:
0.117 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 马可罗索(KL)酶联免疫吸附检测试剂盒 |
| 英文名称 | Horse KL(Klotho) ELISA Kit |
| 别名 | Kl ; KLOT; Klotho peptide |
| 货号 | ELK9557 |
| 反应种属 | Horse |
| 检测类型 | Sandwich |
| 灵敏度 | 0.117 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | Serum, plasma, tissue homogenates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse KL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse KL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse KL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse KL in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Metabolic pathway; |

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文献和实验相关实验
输血的深入开展,对HPA分型的需求在日益增长。血清学方法,如混合被动血凝试验(MPHA)、血小板抗原单克隆特异性抗体固定试验(MAIPA)、酶联免疫吸附试验(ELISA)以及简易致敏红细胞血小板血清学试验(SEPSA)等于血小板抗体的检测固然比较简便,但用于HPA定型则有其不足之处:如血小板抗体血清很难获得,尤其是抗2a和抗3b血清,而且在一些PTP或PTR病人中较难获得足够的血小板用于血清学试验。近来开始出现的DNA分型方法则克服了这些不足,它不需要血小板抗体和新鲜的血小板,只需要少量的全血或组织便
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马可罗索(KL)酶联免疫吸附检测试剂盒【Horse KL(Klotho) ELISA Kit】
¥1960 - 2800





