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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
15.63-1000 pg/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
6.3 pg/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人可溶性核因子κB受体激活因子配体(sRANkL)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human sRANkL(Soluble Receptor Activator Of Nuclear Factor Kappa B Ligand) ELISA Kit |
| 别名 | CD254; TNFSF11; ODF; OPGL; TRANCE; HRANKL2; SOdf; Tumor Necrosis Factor(ligand)superfamily Member 11; TNF-related activation-induced cytokine |
| 货号 | ELK9523 |
| 反应种属 | Human |
| 检测类型 | Sandwich |
| 灵敏度 | 6.3 pg/mL |
| 标准品 | 1000 pg/mL |
| 检测范围 | 15.63-1000 pg/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sRANkL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sRANkL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sRANkL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sRANkL in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction;Metabolic pathway;Apoptosis;Tumor immunity;Bone metabolism; |

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文献和实验Receptor Binding Assay for NO-Independent Activators of Soluble Guanylate Cyclase
receptor binding assay that was successfully applied to determine the binding constant of the NO-independent activator of soluble guanylate cyclase, cinaciguat, and the impact of other small molecules on its interaction with the enzyme.
To assess the role of sortilin in the sorting and trafficking of sphingolipid activator proteins (SAPs) the function of sortilin was abolished by a dominant-negative mutant and by the use of RNAi. Mutant sortilin lacking the carboxyl-terminal
activation motif; JAK, Janus kinase; NFκB, nuclear factor κB; PKB, protein kinase B; PLC, phospholipase C; SIGIRR, single Ig IL-1-related receptor; sIL-1RAcP, soluble IL-1 receptor accessory protein; sST2, soluble ST2; STAT3, signal transducer and activator
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