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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
78.13-5000 pg/mL
- 检测方法:
Sandwich
- 适应物种:
Rat
- 样本:
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
37 pg/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1680.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2400.0 |
| 中文名称 | 大鼠白细胞分化抗原CD4(CD4)酶联免疫吸附检测试剂盒 |
| 英文名称 | Rat CD4(Cluster Of Differentiation 4) ELISA Kit |
| 别名 | CD4mut; p55; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD4; Cluster Of Differentiation 4 |
| 货号 | ELK9500 |
| 反应种属 | Rat |
| P05540 | P05540 |
| 检测类型 | Sandwich |
| 灵敏度 | 37 pg/mL |
| 标准品 | 5000 pg/mL |
| 检测范围 | 78.13-5000 pg/mL |
| 样本类型 | tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CD4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CD4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CD4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CD4 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction;CD & Adhesion molecule;Immune molecule; |

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文献和实验大鼠CD4分子(CD4) 酶联免疫 分析( ELISA ) 试剂 盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定大鼠血清,血浆及相关液体样本中 CD4分子(CD4) 含量。 实验原理 : 本试剂盒应用双抗体夹心法测定 标本 中 大鼠 CD4分子( CD4 ) 水平。用纯化的 大鼠 CD4分子( CD4 ) 抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入 CD4分子( CD4 ) ,再与HRP
分析法,将识别同一分化抗原的来自不同实验室的单克隆抗体归为一个分化群,简称CD(cluster of differentiation)。在许多场合下,抗体及其识别的相应抗原都用同一个CD序号,因此在参阅教科书和文献时需加注意。 一、人白细胞分化抗原的分类 迄今为至,人CD的序号已从CD1命名至CDw130(见表1-2),可大致划分为T细胞、B细胞、髓系细胞、NK细胞、血小板、激活抗原、粘附分子、内皮细胞和细胞因子受体等九个组(表1-1)。 表1-1 CD单抗分组(1993
, dendritic cells, granulocytes Positive staining (disease): pityriasis lichenoides Micro images: acute demyelinating disease, extranodal NK/T cell lymphoma, nasal type ELISA KIT & ANTIBODY OF USCNLIFE
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