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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
15.63-1000 pg/mL
- 检测方法:
Sandwich
- 适应物种:
Horse
- 样本:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
6 pg/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 马心肌肌钙蛋白T(cTnT/TNNT2)酶联免疫吸附检测试剂盒 |
| 英文名称 | Horse cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit |
| 别名 | CMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T; TNNT2; Troponin T Type 2, Cardiac |
| 货号 | ELK9455 |
| 反应种属 | Horse |
| 检测类型 | Sandwich |
| 灵敏度 | 6 pg/mL |
| 标准品 | 1000 pg/mL |
| 检测范围 | 15.63-1000 pg/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse cTnT/TNNT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse cTnT/TNNT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse cTnT/TNNT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse cTnT/TNNT2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Cardiovascular biology; |

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文献和实验. When we use these specific oligonucleotides in a PCR, we generate a 366 bp amplification product from the wild-type DNA (T-target) and a 473 bp amplification product from the competitor DNA (Ccompetitor) (Fig. 1 b ). The PCR reactions were set up in 50 µl
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3' NdeI Type II restriction enzyme 5' CA^TATG 3' NheI Type II restriction enzyme 5' G^CTAGC
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