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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
15.63-1000 pg/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Tissue homogenates, cell lysates and other biological fluids.
- 灵敏度:
5.5 pg/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人少突胶质细胞系转录因子2(OLIG2)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human OLIG2(Oligodendrocyte Lineage Transcription Factor 2) ELISA Kit |
| 别名 | BHLHB1; OLIGO2; PRKCBP2; RACK17; Basic Domain,Helix-Loop-Helix Protein,Class B 1; Class B basic helix-loop-helix protein 1; Class E basic helix-loop-helix protein 19 |
| 货号 | ELK9367 |
| 反应种属 | Human |
| Q13516 | Q13516 |
| 检测类型 | Sandwich |
| 灵敏度 | 5.5 pg/mL |
| 标准品 | 1000 pg/mL |
| 检测范围 | 15.63-1000 pg/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OLIG2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OLIG2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human OLIG2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OLIG2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Neuro science; |

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文献和实验Culturing Oligodendrocyte Lineage Cells from Neonatal Rats
The use of enriched oligodendrocyte lineage cell cultures has yielded insight into functions of these cells and regulatory mechanisms. This chapter details methods that result in such cultures.
Derivation of Oligodendrocyte Progenitor Cells from Human Embryonic Stem Cells
. Here, we describe a protocol to generate oligodendroglial lineage-specific cells in high purity from human embryonic stem cells.
have not been purified, limiting the ability to study the earliest stages of commitment to the oligodendrocyte lineage. Pluripotent mouse embry-onic stem (ES) cells can be propagated indefinitely in culture and induced to differentiate into various cell types
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