- ￥1960 - 2800
- ELK Biotechnology
- 78.13-5000 pg/mL
- 27 pg/mL
- 武汉
- ELK9330MS
- 2026年04月25日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
78.13-5000 pg/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
Serum, plasma, tissue homogenates and other biological fluids.
- 灵敏度：
27 pg/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1960.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2800.0 |
| 中文名称 | 人CD177分子(CD177)微量上样酶联免疫吸附检测试剂盒 |
| 英文名称 | Human CD177(CD177 antigen) Microsample ELISA Kit |
| 别名 | CD177; NB1; PRV1; CD177 antigen; Human neutrophil alloantigen 2a; HNA-2a; NB1 glycoprotein; NB1 GP; Polycythemia rubra vera protein 1; PRV-1; CD177 |
| 货号 | ELK9330MS |
| 反应种属 | Human |
| Q8N6Q3 | Q8N6Q3 |
| 检测类型 | Sandwich |
| 灵敏度 | 27 pg/mL |
| 标准品 | 5000 pg/mL |
| 检测范围 | 78.13-5000 pg/mL |
| 样本类型 | Serum, plasma, tissue homogenates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD177. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD177. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD177, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD177 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction; |
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的酶活力直接反映游离的酶标记物。均相EIA在临床检验中较少应用。非均相EIA需先进行游离的和结合的标记物的分离。如前所述，固相载体可用作一种分离手段。这种固相酶免疫测定方法在1971年最初建立时称为酶联免疫吸附剂测定（enzyme linked immunosorbent assay），简称ELISA，在国内有译作酶联免疫吸附试验或酶标，已习用。2、ELISA的原理和类型2.1. ELISA的原理ELISA的基础是抗原或抗体的固相化及抗原或抗体的酶标记。结合在固相载体表面的抗原或抗体仍保持其免疫
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