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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
15.63-1000 pg/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
serum, plasma, tissue homogenates, cell lysates and other biological fluids
- 灵敏度:
6.6 pg/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1680.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2400.0 |
| 中文名称 | 人可溶性CD40配体(sCD40L)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human sCD40L(Soluble Cluster Of Differentiation 40 Ligand) ELISA Kit |
| 别名 | CD154; TRAP; HIGM1; IGM; IMD3; TBAM; T-BAM; TNFSF5; Gp39; TNF Superfamily Member 5; Hyper-IgM Syndrome; TNF-Related Activation Protein; T-Cell B-Cell Activating Molecule |
| 货号 | ELK9196 |
| 反应种属 | Human |
| 检测类型 | Sandwich |
| 灵敏度 | 6.6 pg/mL |
| 标准品 | 1000 pg/mL |
| 检测范围 | 15.63-1000 pg/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sCD40L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sCD40L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sCD40L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sCD40L in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction;CD & Adhesion molecule;Apoptosis;Tumor immunity;Infection immunity;Immune molecule; |

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