兔核因子κB亚基p105(NFKB-p105)酶联免疫吸附检
测试剂盒【Rabbit NF-κB p105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit】- ￥1960 - 2800
- ELK Biotechnology
- 0.32-20 ng/mL
- 0.113 ng/mL
- 武汉
- ELK9180
- 2026年04月25日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
0.32-20 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Rabbit
- 样本：
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度：
0.113 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1960.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2800.0 |
| 中文名称 | 兔核因子κB亚基p105(NFKB-p105)酶联免疫吸附检测试剂盒 |
| 英文名称 | Rabbit NF-κB p105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit |
| 别名 | NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit; NFkB; Nuclear Factor Kappa B |
| 货号 | ELK9180 |
| 反应种属 | Rabbit |
| 检测类型 | Sandwich |
| 灵敏度 | 0.113 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit NF-κBp105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit NF-κBp105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit NF-κBp105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit NF-κBp105 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction; |
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文献和实验Activation of Nuclear Factor-B (NF-B) in Mononuclear Cells (MNC)
The transcription factor NF-κB was first identified as a regulator of the expression of the kappa light-chain gene in murine B lymphocytes (1 ). It has a central role in the immunological processes and regulates a wide range of genes
xueruo 你好，本人要做IKK-NF-κB通路方面的研究，做的是胃上皮细胞，指导导师让我用NBD抑制IKK活性，并要求我订购IKKβ磷酸化抗体做IKK活性试验，我有点困惑，查了不少的文献，IKK都是引起IκB磷酸化激活NF-κB的，而非IKK磷酸化激活的，其中的原理是什么?NBD多肽我在文献上看到有野生型，突变型，好多文献里NBD多肽是找公司合成的，肽链有长有短，打电话问公司订，他们要求我提供序列，我也不知道提供什么？恳请各位大侠给出高见，谢谢
The nuclear factor-kappa B (NFκB) family of transcription factors has emerged as a signaling pathway that figures prominently in a cell’s initial response to a plethora of inflammatory stimuli. Modified lipids, oxidative stress, bacterial
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