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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Rat
- 样本:
Tissue homogenates, cell lysates and other biological fluids
- 灵敏度:
0.068 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 大鼠含胶原三螺旋重复蛋白1(CTHRC1)酶联免疫吸附检测试剂盒 |
| 英文名称 | Rat CTHRC1(Collagen Triple Helix Repeat Containing Protein 1) ELISA Kit |
| 别名 | NMTC1 |
| 货号 | ELK9170 |
| 反应种属 | Rat |
| Q8CG08 | Q8CG08 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.068 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CTHRC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CTHRC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CTHRC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CTHRC1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction; |

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文献和实验Stabilization of Collagen-Model, Triple-Helical Peptides for In Vitro and In Vivo Applications
The triple-helical structure of collagen has been accurately reproduced in numerous chemical and recombinant model systems. Triple-helical peptides and proteins have found application for dissecting collagen-stabilizing forces
Quantitative Determination of Collagen Crosslinks
. These links are formed by oxidative deamination of the ε-amino group of the single lysine in the amino and carboxy-telopeptides by lysyl oxidase. The aldehyde thus formed reacts with an ε-amino group of a lysine at a specific point in the triple helix
Determination of Collagen Content, Concentration, and Sub-types in Kidney Tissue
content and concentration. Based on the method of Bergman and Loxley (8 ), this spectrophotometric technique estimates total collagen by measuring the hydroxyproline content of tissue. The assay relies on the fact that the collagen triple helix
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