人腺瘤性结肠息肉病蛋白(APC)微量上样酶联免疫吸附检测试剂
盒【Human APC(Adenomatous polyposis coli) Microsample ELISA Kit】- ￥1820 - 2600
- ELK Biotechnology
- 15.63-1000 pg/mL
- 5.5 pg/mL
- 武汉
- ELK9156MS
- 2026年04月25日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
15.63-1000 pg/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
Serum, plasma, tissue homogenates and other biological fluids
- 灵敏度：
5.5 pg/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1820.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2600.0 |
| 中文名称 | 人腺瘤性结肠息肉病蛋白(APC)微量上样酶联免疫吸附检测试剂盒 |
| 英文名称 | Human APC(Adenomatous polyposis coli) Microsample ELISA Kit |
| 别名 | APC; DP2; DP3; FPC; GS; DP2.5; Protein APC; Min; adenomatosis polyposis coli; adenomatosis polyposis coli tumor suppressor; adenomatous polyposis coli; adenomatous polyposis coli protein; BTPS2; Deleted in polyposis 2.5 |
| 货号 | ELK9156MS |
| 反应种属 | Human |
| P25054 | P25054 |
| 检测类型 | Sandwich |
| 灵敏度 | 5.5 pg/mL |
| 标准品 | 1000 pg/mL |
| 检测范围 | 15.63-1000 pg/mL |
| 样本类型 | Serum, plasma, tissue homogenates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human APC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APC in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Cell Biology; Neuroscience; Epigenetics and Nuclear Signaling |
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文献和实验酶活性的DNA聚合酶，原来通常使用的是Taq DNA聚合酶，缺少校正功能。在这儿我们展示将长PCR技术应用到5＇RACE系统中，来分离5.5kb长的人结节性脑硬化II（tuberous sclerosis II，TSC-2）mRNA和8.9kb长的腺瘤性结肠息肉（adenomatous polyposis coli，APC）mRNA的5＇末端，扩增的5＇末端片段要比单独用Taq DNA聚合酶获得的片段长。方法使用5＇RACE系统版本2 （Cat. No. 18374-058）来扩增TSC-2和APC
The APC Tumor Suppressor Pathway
Colon cancer is the second most common cause of cancer mortality among adults in the United States today (1 ). Colon cancer arises from a pathological transformation of the normal colonic epithelium to an adenomatous polyp
Assay for Detecting the I1307K Susceptibility Allele within the Adenomatous Polyposis ColiGene
Most germline mutations of the adenomatous polyposis coli (APC ) tumor suppressor gene result in a classic inherited cancer syndrome called familial adenomatous polyposis (FAP). FAP is characterized by thousands of colonic polyps
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