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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
15.63-1000 pg/mL
- 检测方法:
Sandwich
- 适应物种:
Sheep
- 样本:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
5.5 pg/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 绵羊白介素18(IL18)酶联免疫吸附检测试剂盒 |
| 英文名称 | Sheep IL18(Interleukin 18) ELISA Kit |
| 别名 | IL-18;IGIF; IL-1g; IL1F4; IL1-F4; Interferon-Gamma-Inducing Factor; Interleukin-1 Family Member 4; Iboctadekin; Interleukin-1 Gamma |
| 货号 | ELK9132 |
| 反应种属 | Sheep |
| 检测类型 | Sandwich |
| 灵敏度 | 5.5 pg/mL |
| 标准品 | 1000 pg/mL |
| 检测范围 | 15.63-1000 pg/mL |
| 样本类型 | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep IL18. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep IL18. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep IL18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep IL18 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Cytokine;Infection immunity; |

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文献和实验相关专题 &NBS p; Interleukin 18 (interferon-gamma-inducing factor) IL-18 is a cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other Cell s. IL-18 works by binding
ELATNESCLASRETSFITNG HCLASGKTSF MTTLCLRSIY EDLKMYHVEF QAMNAKLLMDPKRQIFLDQN MLAAIAELMQ ALNFDSETVP QKPSLKELDF YKTKVKLCILLHAFRIRAVT IDRMMSYLSS S2, FeaturesDL-IL12A-Ra大鼠白介素 12A (IL12A) 酶联免疫吸附检测试剂盒预期应用本试剂盒运用双抗体夹心 ELISA 法定量测定大鼠血清、血浆、组织匀浆、细胞裂解液、细胞培养上清液和其他生物液体中 IL
「魔高一尺道高一丈」周挺等 Nature 报道分泌型免疫检查点,伪 IL-18 肿瘤免疫治疗异军突起
样本进行了分析,TCGA 数据库分析发现 IL18-BP 在多种肿瘤类型中表达均升高。此外,IL-18BP 的表达与 CD3E,CD8A 和 PDCD1 的表达高度相关,提示其表达的增加与活化的 CD8+ T 细胞的存在有关。免疫组化和 ELISA 的结果也证实 IL-18BP 在肿瘤组织或血清中高表达。进一步地小鼠实验发现,mIL-18 对野生型小鼠的肿瘤生长没有影响,但能显著抑制 IL-18BP 敲除小鼠的肿瘤生长。这些结果提示 IL-18BP 的表达在癌症中很常见,并且可以充当可溶性免疫
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