人性别决定区Y框蛋白10(SOX10)酶联免疫吸附检测试剂盒
【Human SOX10(Sex Determining Region Y Box Protein 10) ELISA Kit】- ￥1820 - 2600
- ELK Biotechnology
- 0.16-10 ng/mL
- 0.048 ng/mL
- 武汉
- ELK9120
- 2026年04月25日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
0.16-10 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
Tissue homogenates, cell lysates and other biological fluids
- 灵敏度：
0.048 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1820.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2600.0 |
| 中文名称 | 人性别决定区Y框蛋白10(SOX10)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human SOX10(Sex Determining Region Y Box Protein 10) ELISA Kit |
| 别名 | DOM; MGC15649; SOX 10; SRY (sex determining region Y) box 10; SRY box 10; SRY box containing gene 10; SRY related HMG box gene 10 |
| 货号 | ELK9120 |
| 反应种属 | Human |
| 检测类型 | Sandwich |
| 灵敏度 | 0.048 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SOX10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SOX10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SOX10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SOX10 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Neuro science |
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文献和实验Protein Techniques: Immunoprecipitation, In Vitro Kinase Assays, and Western Blotting
. For example, the introduction of a portion of the Y chromosome into a female mouse can change its sex, demonstrating the presence of a sex-determining gene (1 ). In combination with a range of molecular biology techniques, such as RNA protection, PCR
/ml DNase‐free RNase A (see recipe ) 10× loading buffer (see recipe ) Primary antibody against protein or epitope
Application of Amide Proton Exchange Mass Spectrometry for the Study of Protein‐Protein Interactions
Basic Protocol 1: Identification of the Protein‐Protein Interface Via the On‐Exchange Method Materials 10× low‐salt
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