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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
15.63-1000 pg/mL
- 检测方法:
Sandwich
- 适应物种:
Rat
- 样本:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
7.4 pg/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 大鼠含PYD和CARD域蛋白(PYCARD)酶联免疫吸附检测试剂盒 |
| 英文名称 | Rat PYCARD(PYD And CARD Domain Containing Protein) ELISA Kit |
| 别名 | ASC; CARD5; TMS1; Apoptosis-associated speck-like protein containing a CARD; Caspase recruitment domain-containing protein 5; Target of methylation-induced silencing 1 |
| 货号 | ELK8931 |
| 反应种属 | Rat |
| 检测类型 | Sandwich |
| 灵敏度 | 7.4 pg/mL |
| 标准品 | 1000 pg/mL |
| 检测范围 | 15.63-1000 pg/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PYCARD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PYCARD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PYCARD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PYCARD in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Apoptosis; |

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文献和实验TA、HET-E和TPl。 (3)C端为效应结构域。主要由两种成分组成:胱天蛋白酶招募结构域(caspase recruitment domain,CARD)或热蛋白结构域(pyrin domain,PYD),功能是把NLR受体分子和下游衔接蛋白及效应分子连接起来。在LRR和NACHT中间还可以有一个NACHT相关结构域(NACHT-associated domain,NAD)。在C端也可以接上另一种效应结构域,称为BIR。 ANLR分子结构示意图 NOD指结合核苷酸的寡聚
炎症促发阿尔茨海默病又添一力证!NLRP3 炎症小体激活引发 tau 蛋白异常
regeneration,ALR)蛋白家族作为受体蛋白(receptor)、凋亡相关微粒蛋白(apoptosis-associated speck-like protein containing CARD)ASC 作为接头蛋白(adaptor)、含半胱氨酸的天冬氨酸蛋白水解酶 caspase 作为效应蛋白(effector)。其中接头蛋白 ASC 包含两个重要的结构域即 PYD 和 CARD。CARD 结构域发挥桥梁作用,连接受体蛋白和效应蛋白,ASC 的 CARD 结构域可与效应蛋白 CARD 结构域结合形成
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