- ￥1960 - 2800
- ELK Biotechnology
- 15.63-1000 pg/mL
- 6.2 pg/mL
- 武汉
- ELK8802
- 2026年04月25日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
15.63-1000 pg/mL
- 检测方法：
Sandwich
- 适应物种：
Cattle
- 样本：
Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
- 灵敏度：
6.2 pg/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1960.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2800.0 |
| 中文名称 | 牛内皮型一氧化氮合酶(NOS3)酶联免疫吸附检测试剂盒 |
| 英文名称 | Cattle NOS3(Nitric Oxide Synthase 3, Endothelial) ELISA Kit |
| 别名 | ECNOS; NOS III; E-NOS; C-NOS; ENOS; CNOS; Constitutive NOS |
| 货号 | ELK8802 |
| 反应种属 | Cattle |
| P29473 | P29473 |
| 检测类型 | Sandwich |
| 灵敏度 | 6.2 pg/mL |
| 标准品 | 1000 pg/mL |
| 检测范围 | 15.63-1000 pg/mL |
| 样本类型 | Serum, plasma, tissue homogenates, cell lysates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle NOS3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle NOS3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle NOS3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle NOS3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Enzyme & Kinase;Cardiovascular biology; |
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文献和实验-dependent and constitutively expressed and produce low levels of NO: NOS1 (neuronal NOS or nNOS), which is found mostly in neurons and skeletal muscle, and NOS3 (endothelial NOS or eNOS), which is found mostly in endothelial cells. NOS1 is critical
分析，并选取部分筛选出的差异表达miRNA，以半定量逆转录 聚合酶链反应（RT PCR）和Western blot分析其相对应的靶mRNA和目的蛋白表达状况。作者认为液相芯片筛选差异表达miRNA可为研究HCC发病机制和诊断治疗提供新的思路。 3.6 研究细胞信号转导途径 Mu［19］为研究resistin是否对人内皮细胞有直接作用，将人冠状动脉内皮细胞（HCAEC）用无血清培养基培养后，用resistin处理，然后取细胞裂解液用Bio Rad公司的“磷酸化蛋白和总靶蛋白检测试剂盒
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