- ￥1820 - 2600
- ELK Biotechnology
- 0.16-10 ng/mL
- 0.062 ng/mL
- 武汉
- ELK8794
- 2026年04月25日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
0.16-10 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Rat
- 样本：
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度：
0.062 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1820.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2600.0 |
| 中文名称 | 大鼠细胞程序性死亡蛋白1(PDCD1)酶联免疫吸附检测试剂盒 |
| 英文名称 | Rat PDCD1(Programmed Cell Death Protein 1) ELISA Kit |
| 别名 | CD279; PD1; SLEB2; HPD1 |
| 货号 | ELK8794 |
| 反应种属 | Rat |
| 检测类型 | Sandwich |
| 灵敏度 | 0.062 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PDCD1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PDCD1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PDCD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PDCD1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Apoptosis;Immune molecule;Endocrinology;Autoimmunity; |
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文献和实验酶免疫测定（enzyme immunoassay, EIA）或免疫酶技术（immunoenzymatic technique）是指用酶标记抗体或酶标记抗体进行的抗原抗体反应。它采用抗原与抗体的特异反应与酶连接，然后通过酶与底物产生颜色反应，用于定量测定。目前常用的方法称为酶联免疫吸附法（enzyme-linked immunosorbent assay, ELISA），其方法简单，方便讯速，特异性强。ELISA是由抗原（抗体）先结合在固相
ELATNESCLASRETSFITNG HCLASGKTSF MTTLCLRSIY EDLKMYHVEF QAMNAKLLMDPKRQIFLDQN MLAAIAELMQ ALNFDSETVP QKPSLKELDF YKTKVKLCILLHAFRIRAVT IDRMMSYLSS S2, FeaturesDL-IL12A-Ra大鼠白介素 12A （IL12A） 酶联免疫吸附检测试剂盒预期应用本试剂盒运用双抗体夹心 ELISA 法定量测定大鼠血清、血浆、组织匀浆、细胞裂解液、细胞培养上清液和其他生物液体中 IL
The following video tutorial described how to run a sandwich ELISA, also known as enzyme-linked immunosorbent assay, using a general PeproTech protocol. 接下来的实验指导讲述PeproTech夹心法ELISA，即酶联免疫吸附试验的通用步骤 The capture, standard, and detection are supplied
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