小鼠跨膜蛋白119(TMEM119)微量上样酶联免疫吸附检测
试剂盒【Mouse TMEM119(Transmembrane Protein 119) Microsample ELISA Kit】- ￥1960 - 2800
- ELK Biotechnology
- 0.16-10 ng/mL
- 0.054 ng/mL
- 武汉
- ELK8672MS
- 2026年04月25日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
0.16-10 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Mouse
- 样本：
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度：
0.054 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1960.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2800.0 |
| 中文名称 | 小鼠跨膜蛋白119(TMEM119)微量上样酶联免疫吸附检测试剂盒 |
| 英文名称 | Mouse TMEM119(Transmembrane Protein 119) Microsample ELISA Kit |
| 别名 | PSEC0199;OBIF |
| 货号 | ELK8672MS |
| 反应种属 | Mouse |
| Q8R138 | Q8R138 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.054 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TMEM119. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TMEM119. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TMEM119, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TMEM119 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction; |
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文献和实验Genome Oligo Set，包含13971个来源于UniGene中的已知基因对应的70-mer寡核苷酸序列（Hs build 119）和29个对照。（The UniGene database automatically clusters all human Genbank sequences into a non-redundant set of genes. Similar databases are also available for the rat and mouse genomes
，平均批间变异系数为11.4％；液相芯片与ELISA法检测结果的相关系数R2为0.88～0.99。 国外多家公司已推出了商品化的细胞因子液相芯片检测试剂盒，比如R&D 公司的Flurokine MAP系列、Linco Research公司的LINCOplex 试剂盒、BioSource公司的Antibody Bead Kit、Bio Rad公司的Bio Plex系列等，可用于来自人、小鼠、大鼠的样品，既有一次检测单种细胞因子的试剂盒，也有预先混合多种细胞因子抗体交联微球而一次检测多个指标
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