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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.63-40 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Serum, plasma, tissue homogenates and other biological fluids.
- 灵敏度:
0.261 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 人催乳素诱导蛋白(PIP)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human PIP(Prolactin Induced Protein) ELISA Kit |
| 别名 | GCDFP15; GPIP4; BRST2; SABP; gp17; Gross cystic disease fluid protein 15; Prolactin-induced protein; Secretory actin-binding protein |
| 货号 | ELK6287 |
| 反应种属 | Human |
| P12273 | P12273 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.261 ng/mL |
| 标准品 | 40 ng/mL |
| 检测范围 | 0.63-40 ng/mL |
| 样本类型 | Serum, plasma, tissue homogenates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PIP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PIP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PIP in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Immune molecule; |

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文献和实验yys700714 请问如何用免疫沉淀法测PI3K活性呢?我查到相关文献,写得很清楚的,不过还是不太懂,不知需要准备什么试剂,需要什么仪器测定,TLC是什么啊,是不是需要荧光图像仪?很多疑问,请求做过的高手给予指点。多谢!! yhwangcams 建议可以使用Echelon的PI3-K ELISA试剂盒。 http://www.echelon-inc.com/ccp1231-pi3-k-elisa-kit-k
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