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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Serum, plasma, tissue homogenates and other biological fluids.
- 灵敏度:
0.054 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人C-Ros癌基因1(ROS1)微量上样酶联免疫吸附检测试剂盒 |
| 英文名称 | Human ROS1(C-Ros Oncogene 1, Receptor Tyrosine Kinase) Microsample ELISA Kit |
| 别名 | MCF3; ROS; c-Ros-1; v-Ros Avian UR2 Sarcoma Virus Oncogene Homolog 1; Proto-oncogene tyrosine-protein kinase ROS; Receptor tyrosine kinase c-ros oncogene 1 |
| 货号 | ELK5050MS |
| 反应种属 | Human |
| P08922 | P08922 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.054 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Serum, plasma, tissue homogenates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ROS1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ROS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ROS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ROS1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Tumor immunity; |

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文献和实验-3-3 associates with several tyrosine-phosphorylated proteins and phosphatidylinositol 3-kinase (PI3-K) in T cells. We report here the identification of the 120-kDa 14-3-3tau-binding phosphoprotein present in activated T cell lysates as Cbl, a protooncogene
Introduction of human 14-3-3 proteins
whose members have been shown to activate tyrosine and tryptophan hydroxylases,inhibit protein kinase C and possess phospholipase A2 activity in vitro.We have isolated and analyzed a 14-3-3 protein cDNA clone (H14-3-3)from a human fetal brain cDNA library
Gene (Ref) Accession # Position from ATG Gene Target (Comments) Cell Line Tested ß-actin (1) NM_001101 972-994 aatgaagatcaagatcattgc Human HeLa gamma-actin (1) NM_001614 8-30 aagagatcgccgcgctggtca Human HeLa AIB1 (estrogen receptor
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