大鼠过氧化物酶体增殖物激活受体α(PPARa)酶联免疫吸附检
测试剂盒【Rat PPARa(Peroxisome Proliferator Activated Receptor Alpha) ELISA Kit】- ￥1820 - 2600
- ELK Biotechnology
- 0.32-20 ng/mL
- 0.11 ng/mL
- 武汉
- ELK4576
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
0.32-20 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Rat
- 样本：
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度：
0.11 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1820.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2600.0 |
| 中文名称 | 大鼠过氧化物酶体增殖物激活受体α(PPARa)酶联免疫吸附检测试剂盒 |
| 英文名称 | Rat PPARa(Peroxisome Proliferator Activated Receptor Alpha) ELISA Kit |
| 别名 | NR1C1; PPAR-A; HPPAR; Nuclear Receptor Subfamily 1 Group C Member 1 |
| 货号 | ELK4576 |
| 反应种属 | Rat |
| P37230 | P37230 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.11 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PPARa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PPARa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PPARa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PPARa in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction;Tumor immunity;Developmental science; |
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文献和实验PPAR-Alpha Cloning, Expression, and Characterization
Peroxisome proliferator-activated receptor α (PPARα) is a member of the nuclear/steroid receptor gene superfamily that also comprises β, δ, and γ isoforms. PPARα is a ligand-activated transcription factor that plays
Analyzing PPAR/Ligand Interactions by Chemical Cross-Linking and High-Resolution Mass Spectrometry
for gaining insights into the conformational changes of the peroxisome proliferator-activated receptor alpha after binding of low-molecular weight ligands. Our strategy provides a basis to efficiently characterize target protein-drug
The capacity to induce the association of peroxisome proliferator-activated receptors (PPARs) with different transcriptional coregulators is determined by the peculiar 3D-structure that the receptors adopt when bound with a specific ligand
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