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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.32-20 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Serum, plasma, tissue homogenates and other biological fluids
- 灵敏度:
0.139 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人胰岛素降解酶(IDE)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human IDE(Insulin Degrading Enzyme) ELISA Kit |
| 别名 | Insulysin; Insulin Protease; Abeta-degrading protease; Insulinase |
| 货号 | ELK2906 |
| 反应种属 | Human |
| P14735 | P14735 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.139 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | Serum, plasma, tissue homogenates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IDE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IDE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IDE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IDE in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Enzyme & Kinase;Metabolic pathway;Endocrinology;Cardiovascular biology; |

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文献和实验CUSABIO月度文献盘点:5月新增200篇产品引用文献,总文献数量已达 31200篇!
dissection 影响因子:13.3 发表期刊:Theranostics CUSABIO助力产品: Human activated protein C,APC ELISA Kit; CSB-E09909hHuman Insulin,INS ELISA Kit; CSB-E05069hMouse activated protein C,APC ELISA Kit; CSB-E09914mMouse Insulin,INS ELISA Kit; CSB-E05071m 研究亮点 本研究聚焦主动
1179380155 请问能用ELISA(酶连接免疫吸附)法检测基因突变吗?感激不尽! woxingwosu ELISA-based Ki-ras gene mutation analyses in pancreatic and cholangiocarcinoma cells and tissues. Abstract Detection of Ki-ras mutations
的酶活力直接反映游离的酶标记物。均相EIA在临床检验中较少应用。非均相EIA需先进行游离的和结合的标记物的分离。如前所述,固相载体可用作一种分离手段。这种固相酶免疫测定方法在1971年最初建立时称为酶联免疫吸附剂测定(enzyme linked immunosorbent assay),简称ELISA,在国内有译作酶联免疫吸附试验或酶标,已习用。2、ELISA的原理和类型2.1. ELISA的原理ELISA的基础是抗原或抗体的固相化及抗原或抗体的酶标记。结合在固相载体表面的抗原或抗体仍保持其免疫
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