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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
39.07-2500 pg/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
15 pg/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1680.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2400.0 |
| 中文名称 | 人氨基端前脑钠素(NT-ProBNP)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit |
| 别名 | NT-Pro-BNP; ; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb |
| 货号 | ELK1212 |
| 反应种属 | Human |
| 检测类型 | Sandwich |
| 灵敏度 | 15 pg/mL |
| 标准品 | 2500 pg/mL |
| 检测范围 | 39.07-2500 pg/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Endocrinology;Cardiovascular biology; |

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力后,BNP就开始由心肌细胞合成,但是并不是直接合成BNP,而是先合成pro-BNP(可以翻译成前脑钠肽或者脑钠肽原),pro-BNP具有108个氨基酸,在血液中会分解,产生BNP,分解下来的片段是其N端的76个氨基酸,这76个氨基酸就是NT-proBNP ,也就是经常检测的那个指标,剩下的32个氨基酸才叫BNP。没有NT-BNP的概念,所谓的NT-BNP应该是指NT-proBNP。有点类似于C肽与胰岛素的关系。从这个角度上来讲,都叫脑钠肽不妥,脑钠肽英文应该叫rain(脑) natriuretic(利钠
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