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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.32-20 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Dog
- 样本:
Tissue homogenates, cell lysates and other biological fluids.
- 灵敏度:
0.12 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 犬丝裂原激活蛋白激酶14(MAPK14)酶联免疫吸附检测试剂盒 |
| 英文名称 | Dog MAPK14(Mitogen Activated Protein Kinase 14) ELISA Kit |
| 别名 | CSBP1; CSBP2; CSPB1; EXIP; Mxi2; PRKM14; PRKM15; RK; SAPK2A; P38; Mitogen-activated protein kinase p38 alpha; Cytokine suppressive anti-inflammatory drug-binding protein |
| 货号 | ELK0404 |
| 反应种属 | Dog |
| 检测类型 | Sandwich |
| 灵敏度 | 0.12 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog MAPK14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog MAPK14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog MAPK14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog MAPK14 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction;Enzyme & Kinase;Tumor immunity;Infection immunity; |

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文献和实验Functional Assays on High‐Content Protein Microarrays
Kinase blocking buffer: phosphate‐buffered saline (PBS) containing 1% (w/v) BSA MAPK14/p38γ protein kinase (Invitrogen, cat. no. PV3304
、多结节性及透明质化组织)进行分析,发现肉瘤样区域特异性高表达 TIMP1、C1R 和 TGFBI 等基因,横纹肌样区域则高表达 KIF2A、NAMPT 和 GALNT2。研究还发现,DNA 高甲基化的 Methyl1 亚型与 BAP1 突变及不良预后显著相关,其中 UCHL1 高表达为关键预后标志物,其表达水平与 BAP1 突变、高 WGII 及特定 DNA 甲基化亚型相关,且UCHL1高表达患者预后显著较差。 此外,磷酸化信号通路分析显示,MAPK14-MAPKAPK2 及 EGFR-MEK
ELISPOT(Enzyme-linked Immunospot Assay)全名为酶联免疫斑点检测,结合了细胞培养技术与酶联免疫吸附技术,能够检测到单个细胞分泌的细胞因子情况。简单来说,就是用包被好的抗体捕获培养中的细胞分泌的细胞因子,并以酶联斑点显色的方式将其呈现出来。ELISPOT 实验原理ELISPOT 实验使用 PVDF 膜为底的 96 孔板(cat:MSIPS 4510,Millipore),包被上特异性的单克隆抗体(需要无毒,不含内毒素,亲和力高的抗体),以捕获细胞分泌的细胞
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