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- 文献和实验
- 技术资料
- 服务名称:
大肠杆菌系统重组蛋白表达
- 提供商:
普健生物(武汉)科技有限公司
Uniprot号:P03300
基因名:P2
蛋白名:P2
蛋白别名:
3D, 3D polymerase, 3Dpol, Capsid protein VP0, Capsid protein VP1, Capsid protein VP2, Capsid protein VP3, Capsid protein VP4, EC:2.7.7.48, EC:3.4.22.28, EC:3.4.22.29, EC:3.6.1.15, Genome polyprotein, P1, P1A, P1B, P1C, P1D, P2, P2A, P2B, P2C, P3, P3A, P3B, P3C, Picornain 2A, Picornain 3C, Protease 2A, Protease 3C, Protein 2A, Protein 2B, Protein 2C, Protein 3A, Protein 3AB, Protein 3B, Protein 3CD, Protein 3D, RNA-directed RNA polymerase, RdRp, VP4-VP2, VPg, Viral protein genome-linked, Virion protein 1, Virion protein 2, Virion protein 3, Virion protein 4
服务内容:
| 服务项目 | 客户提供 | 服务内容 | 服务周期 | 交付内容 |
|---|---|---|---|---|
| 大肠杆菌系统 蛋白表达 | 基因序列 | 方案沟通 | 1天 | 方案报告 |
| 密码子优化和基因合成 | 5天 | 基因合成报告 | ||
| 表达纯化测试 | 3天 | 表达纯化测试报告 | ||
| 1L表达及纯化 | 3天 | 蛋白样品(3-5mg)及报告 | ||
| 放大发酵及纯化 | 7天 | 纯化的蛋白及报告 |
案例展示:

P2相关研究文献:
1. Primary structure, gene organization and polypeptide expression of poliovirus RNA. [6264310]
2. Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome. [6272282]
3. Identification of the initiation site of poliovirus polyprotein synthesis. [6284987]
4. The genome-linked protein of picornaviruses. VII. Genetic mapping of poliovirus VPg by protein and RNA sequence studies. [6250717]
5. The 5'-terminal structures of poliovirion RNA and poliovirus mRNA differ only in the genome-linked protein VPg. [202952]
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文献和实验2. Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome. [6272282]
3. Identification of the initiation site of poliovirus polyprotein synthesis. [6284987]
4. The genome-linked protein of picornaviruses. VII. Genetic mapping of poliovirus VPg by protein and RNA sequence studies. [6250717]
5. The 5'-terminal structures of poliovirion RNA and poliovirus mRNA differ only in the genome-linked protein VPg. [202952]
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与患者生存期缩短独立相关;机制上,MOV10通过解旋酶活性直接结合RCMs中的特定基序,解开RCM双链结构,阻断反向剪接,抑制circRNA产生,导致含丰富RCMs侧翼序列的抑癌circRNA下调,进而解除对促癌miRNA的分子海绵效应,抑制下游抑癌蛋白表达,构成MOV10-circRNA-miRNA-抑癌基因的促癌信号轴,促进肝癌细胞增殖、迁移和体内肿瘤生长。该研究为肝癌中circRNA广泛下调提供了全新机制解释,有望为肝癌治疗带来新策略。(图源:10.1038/s41388-026-03869
肝炎病毒包膜E2蛋白相同的亲和结合力3。另外,有阴离子转运载体AE1结构域的重组蛋白被标记且用Rho1D4系统纯化后表现出与红细胞来源的AE1蛋白有相同的硫酸外排速率。4) 利用Rho1D4纯化膜蛋白图解简述步骤1:结合:将Rho1D4标记的蛋白与载有Rho1D4抗体的琼脂糖一同孵育使得目标蛋白被固定在吸附材料上。 步骤2:清洗:将杂蛋白和其他裂解液成分洗去,留下与亲和基质结合的目标蛋白。 步骤3:洗脱:过量Rho1D4肽与基质竞争性结合,释放出目标蛋白在洗脱液中收集。 表
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