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- Monkeypox virus mRNA-capping enzyme catalytic subunit重组蛋白表达
- 2026年04月08日
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- 详细信息
- 文献和实验
- 技术资料
- 服务名称：
大肠杆菌系统重组蛋白表达
- 提供商：
普健生物（武汉）科技有限公司
Uniprot号：A0A7H0DN85
基因名：mRNA-capping enzyme catalytic subunit
蛋白名：mRNA-capping enzyme catalytic subunit
蛋白别名：
EC:2.1.1.56, EC:2.7.7.50, EC:3.6.1.74, GTP--RNA guanylyltransferase, GTase, OPG113, Polynucleotide 5'-triphosphatase, TPase, VTF large subunit, Virus termination factor large subunit, mRNA (guanine-N(7))-methyltransferase, mRNA 5'-triphosphatase, mRNA cap methyltransferase, mRNA guanylyltransferase, mRNA-capping enzyme 97 kDa subunit, mRNA-capping enzyme D1 subunit, mRNA-capping enzyme catalytic subunit, mRNA-capping enzyme large subunit
服务内容：
| 服务项目 | 客户提供 | 服务内容 | 服务周期 | 交付内容 |
|---|---|---|---|---|
| 大肠杆菌系统 蛋白表达 | 基因序列 | 方案沟通 | 1天 | 方案报告 |
| 密码子优化和基因合成 | 5天 | 基因合成报告 | ||
| 表达纯化测试 | 3天 | 表达纯化测试报告 | ||
| 1L表达及纯化 | 3天 | 蛋白样品(3-5mg)及报告 | ||
| 放大发酵及纯化 | 7天 | 纯化的蛋白及报告 |
案例展示：
mRNA-capping enzyme catalytic subunit相关研究文献：
1. Exportation of Monkeypox virus from the African continent. [32880628]
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文献和实验Ribonuclease P (RNase P) from Escherichia coli is a transfer RNA (tRNA)-processing enzyme and consists of a catalytic RNA subunit (M1 RNA) and a protein component (C5 protein). M1GS, a gene-targeting ribozyme derived from M1 RNA, can cleave
In Vitro Selection of RNase P Ribozymes That Efficiently Cleave a Target mRNA
for setting up such a system are included, using the mRNA sequence that encodes the thymidine kinase (TK) of herpes simplex virus 1 (HSV 1) as a target substrate of choice. Using the selection system, we have successfully generated M1GS RNA variants
Synthesis of Double-Stranded Complementary DNA from Poly(A)+mRNA
The use of avian myeloblastosis virus reverse transcriptase (AMV RTase) to produce DNA copies of mRNA templates is a common and well-documented method ( 1 – 3 ). Briefly, the method involves synthesis of a complementary DNA strand to the mRNA
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