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- 详细信息
- 文献和实验
- 技术资料
- 服务名称:
大肠杆菌系统重组蛋白表达
- 提供商:
普健生物(武汉)科技有限公司
Uniprot号:I0DF35
基因名:RNA-directed RNA polymerase L
蛋白名:RNA-directed RNA polymerase L
蛋白别名:
EC:2.7.7.48, EC:3.1.-.-, Large structural protein, Protein L, RNA-directed RNA polymerase L, Replicase, Transcriptase, cap-snatching endonuclease
服务内容:
| 服务项目 | 客户提供 | 服务内容 | 服务周期 | 交付内容 |
|---|---|---|---|---|
| 大肠杆菌系统 蛋白表达 | 基因序列 | 方案沟通 | 1天 | 方案报告 |
| 密码子优化和基因合成 | 5天 | 基因合成报告 | ||
| 表达纯化测试 | 3天 | 表达纯化测试报告 | ||
| 1L表达及纯化 | 3天 | 蛋白样品(3-5mg)及报告 | ||
| 放大发酵及纯化 | 7天 | 纯化的蛋白及报告 |
案例展示:

RNA-directed RNA polymerase L相关研究文献:
1. Fever with thrombocytopenia associated with a novel bunyavirus in China. [21410387]
2. Novel Bunyavirus Infection in China: Preliminary Clinical Characteristics and Treatment Effects. [CI-T9CE162B4QLD]
3. Phylogenetic and Geographic Relationships of Severe Fever With Thrombocytopenia Syndrome Virus in China, South Korea, and Japan. [25762790]
4. Bunyaviridae RdRps: structure, motifs, and RNA synthesis machinery. [28418734]
5. Targeting of severe fever with thrombocytopenia syndrome virus structural proteins to the ERGIC (endoplasmic reticulum Golgi intermediate compartment) and Golgi complex. [29467349]
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文献和实验2. Novel Bunyavirus Infection in China: Preliminary Clinical Characteristics and Treatment Effects. [CI-T9CE162B4QLD]
3. Phylogenetic and Geographic Relationships of Severe Fever With Thrombocytopenia Syndrome Virus in China, South Korea, and Japan. [25762790]
4. Bunyaviridae RdRps: structure, motifs, and RNA synthesis machinery. [28418734]
5. Targeting of severe fever with thrombocytopenia syndrome virus structural proteins to the ERGIC (endoplasmic reticulum Golgi intermediate compartment) and Golgi complex. [29467349]
一般是指 DNA依存性 RNA聚合酶。是催化以 DNA为模板( template)、三磷酸核糖核苷为底物、通过磷酸二酯键而聚合的合成 RNA的酶。因为在细胞内与基因 DNA的遗传信息转录为 RNA有关,所以也称转录酶。反应以下式示: n1 -n4 表示各个模板的 DNA链的胸腺嘧啶、胞嘧啶、鸟嘧啶、腺嘌呤的碱基数。通过特异的碱基配对的形成,合成具有与模板 DNA链完全互补的碱基排列的 RNA。反应是由( 1) DNA与酶的结合
Site-Directed DNA Photoaffinity Labeling of RNA Polymerase III Transcription Complexes
Site-specific DNA photoaffinity labeling is a useful technique for mapping interactions of proteins with DNA in complex systems such as the yeast RNA polymerase III (Pol III) transcription complex, which consists of at least 25 different
Hepatitis C Virus RNA-Dependent RNA Polymerase (NS5B Polymerase)
studies of several recombinant HCV nonstructural proteins have been initiated, our knowledge of the HCV NS5B polymerase that encodes an RNA-dependent RNA polymerase (RDRP) is rudimentary (4 –8 ). RNA-dependent RNA polymerases represent a class of viral
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