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- 详细信息
- 文献和实验
- 技术资料
- 服务名称:
大肠杆菌系统重组蛋白表达
- 提供商:
普健生物(武汉)科技有限公司
Uniprot号:P06663
基因名:Capsid protein
蛋白名:Capsid protein
蛋白别名:
Capsid protein, Coat protein, p38
服务内容:
| 服务项目 | 客户提供 | 服务内容 | 服务周期 | 交付内容 |
|---|---|---|---|---|
| 大肠杆菌系统 蛋白表达 | 基因序列 | 方案沟通 | 1天 | 方案报告 |
| 密码子优化和基因合成 | 5天 | 基因合成报告 | ||
| 表达纯化测试 | 3天 | 表达纯化测试报告 | ||
| 1L表达及纯化 | 3天 | 蛋白样品(3-5mg)及报告 | ||
| 放大发酵及纯化 | 7天 | 纯化的蛋白及报告 |
案例展示:

Capsid protein相关研究文献:
1. Structure and assembly of turnip crinkle virus. IV. Analysis of the coat protein gene and implications of the subunit primary structure. [3612806]
2. The genome structure of turnip crinkle virus. [2718381]
3. HRT gene function requires interaction between a NAC protein and viral capsid protein to confer resistance to turnip crinkle virus. [11041886]
4. The coat protein of turnip crinkle virus suppresses posttranscriptional gene silencing at an early initiation step. [12477856]
5. Turnip crinkle virus coat protein mediates suppression of RNA silencing in Nicotiana benthamiana. [12620795]
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文献和实验2. The genome structure of turnip crinkle virus. [2718381]
3. HRT gene function requires interaction between a NAC protein and viral capsid protein to confer resistance to turnip crinkle virus. [11041886]
4. The coat protein of turnip crinkle virus suppresses posttranscriptional gene silencing at an early initiation step. [12477856]
5. Turnip crinkle virus coat protein mediates suppression of RNA silencing in Nicotiana benthamiana. [12620795]
Construction of Infectious cDNA Clones for RNA Viruses: Turnip Crinkle Virus
system for an RNA virus. Here, we present rapid method for generation of infectious cDNA clone for Turnip crinkle virus (TCV). The infectious cDNA clone could be used for production of in vitro transcripts with the T7 RNA polymerase which could be used for infection
In Situ Hybridization to RNA in Whole Arabidopsis Plants
in plastic wrap, and subjected to autoradiography. The steps before autoradiography require 3–4 d. The exposure time for detection of turnip crinkle virus (TCV) RNA with a 32 P-end-labeled oligonucleotide probe in infected Arabidopsis is as short as 30 min
法和TK基因相比较略显繁琐,需经连续传代,但其不用改造辅助病毒,只需在转移载体中引入Neo基因即可。其它如 PCR、DNA斑点杂交及有限稀释法等,也可用于筛选重组病毒。以上方法各具特点。虽然有些新方法需一定的条件,但一旦建立起来后就可方便、迅速地得到重组病毒,为杆状病毒表达系统的普及应用创造了良好的条件 4、影响外源蛋白表达的因素 利用杆状病毒昆虫表达系统表达外源基因的理论基础,就是杆状病毒的基因表达与调控,但有关病毒晚期基因高表达和其调控机制目前还不十分清楚。利用多角体基因的启动子表达外源
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