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- 详细信息
- 文献和实验
- 技术资料
- 服务名称:
大肠杆菌系统重组蛋白表达
- 提供商:
普健生物(武汉)科技有限公司
Uniprot号:P41658
基因名:Late expression factor 5
蛋白名:Late expression factor 5
蛋白别名:
LEF-5, Late expression factor 5
服务内容:
| 服务项目 | 客户提供 | 服务内容 | 服务周期 | 交付内容 |
|---|---|---|---|---|
| 大肠杆菌系统 蛋白表达 | 基因序列 | 方案沟通 | 1天 | 方案报告 |
| 密码子优化和基因合成 | 5天 | 基因合成报告 | ||
| 表达纯化测试 | 3天 | 表达纯化测试报告 | ||
| 1L表达及纯化 | 3天 | 蛋白样品(3-5mg)及报告 | ||
| 放大发酵及纯化 | 7天 | 纯化的蛋白及报告 |
案例展示:

Late expression factor 5相关研究文献:
1. The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. [8030224]
2. Nucleotide sequence of a gene essential for viral DNA replication in the baculovirus Autographa californica nuclear polyhedrosis virus. [1994581]
3. Identification of genes encoding late expression factors located between 56.0 and 65.4 map units of the Autographa californica nuclear polyhedrosis virus genome. [8249293]
4. AcMNPV late expression factor-5 interacts with itself and contains a zinc ribbon domain that is required for maximal late transcription activity and is homologous to elongation factor TFIIS. [9770426]
5. In vitro activity of the baculovirus late expression factor LEF-5. [12438592]
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文献和实验2. Nucleotide sequence of a gene essential for viral DNA replication in the baculovirus Autographa californica nuclear polyhedrosis virus. [1994581]
3. Identification of genes encoding late expression factors located between 56.0 and 65.4 map units of the Autographa californica nuclear polyhedrosis virus genome. [8249293]
4. AcMNPV late expression factor-5 interacts with itself and contains a zinc ribbon domain that is required for maximal late transcription activity and is homologous to elongation factor TFIIS. [9770426]
5. In vitro activity of the baculovirus late expression factor LEF-5. [12438592]
californica nuclear polyhedrosis virus (AcNPV), are most frequently used because they contain very active late gene promoters for proteins that are expressed late in the virus life cycle and are not essential for virus replication. Replacing a late gene
Use of Baculovirus Expression Vectors
of the expression vector system and the basic biology that underlies it.) Baculoviruses are a large family of DNA viruses that have only been isolated from arthropods, with the majority found in insects. Autographa californica nuclear polyhedrosis virus (AcMNPV
Generation of Recombinant Baculovirus DNA in E.coli Using a Baculovirus Shuttle Vector
of the Autographa californica nuclear polyhedrosis virus (AcNPV) are abundantly expressed during the late stages of infection. The recombinant proteins are usually soluble and functionally similar to their authentic counterparts (l –7 ). In the following sections
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