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- Autographa californica nuclear polyhedrosis virus mRNA capping enzyme LEF-4重组蛋白表达
- 2026年04月08日
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- 详细信息
- 文献和实验
- 技术资料
- 服务名称：
大肠杆菌系统重组蛋白表达
- 提供商：
普健生物（武汉）科技有限公司
Uniprot号：P41477
基因名：mRNA capping enzyme LEF-4
蛋白名：mRNA capping enzyme LEF-4
蛋白别名：
EC:2.7.7.50, EC:3.6.1.74, LEF-4, Late expression factor 4, mRNA capping enzyme LEF-4
服务内容：
| 服务项目 | 客户提供 | 服务内容 | 服务周期 | 交付内容 |
|---|---|---|---|---|
| 大肠杆菌系统 蛋白表达 | 基因序列 | 方案沟通 | 1天 | 方案报告 |
| 密码子优化和基因合成 | 5天 | 基因合成报告 | ||
| 表达纯化测试 | 3天 | 表达纯化测试报告 | ||
| 1L表达及纯化 | 3天 | 蛋白样品(3-5mg)及报告 | ||
| 放大发酵及纯化 | 7天 | 纯化的蛋白及报告 |
案例展示：
mRNA capping enzyme LEF-4相关研究文献：
1. Identification of genes encoding late expression factors located between 56.0 and 65.4 map units of the Autographa californica nuclear polyhedrosis virus genome. [8249293]
2. The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. [8030224]
3. Temporal expression of the AcMNPV lef-4 gene and subcellular localization of the protein. [9499802]
4. A virus-encoded RNA polymerase purified from baculovirus-infected cells. [9733837]
5. RNA 5'-triphosphatase, nucleoside triphosphatase, and guanylyltransferase activities of baculovirus LEF-4 protein. [9811740]
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文献和实验2. The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. [8030224]
3. Temporal expression of the AcMNPV lef-4 gene and subcellular localization of the protein. [9499802]
4. A virus-encoded RNA polymerase purified from baculovirus-infected cells. [9733837]
5. RNA 5'-triphosphatase, nucleoside triphosphatase, and guanylyltransferase activities of baculovirus LEF-4 protein. [9811740]
Introduction to the Molecular Biology of Baculoviruses
of one subgroup, the nuclear polyhedrosis viruses (NPVs). The ability to propagate baculoviruses in cell culture has also allowed extensive study of their molecular biology (10 ). The model virus in these studies is the Autographa californica NPV (AcNPV
Manipulation of Baculovirus Vectors
The prototype baculovirus is the Autographa californica nuclear polyhedrosis virus (AcNPV), which has been the focus for most studies developing expression vector systems. In contrast to other reviews on this topic (1 –3 ) this chapter aims
californica nuclear polyhedrosis virus (AcNPV), are most frequently used because they contain very active late gene promoters for proteins that are expressed late in the virus life cycle and are not essential for virus replication. Replacing a late gene
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