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- 湖北省武汉市
- Autographa californica nuclear polyhedrosis virus DNA replication helicase重组蛋白表达
- 2026年04月08日
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- 详细信息
- 文献和实验
- 技术资料
- 服务名称：
大肠杆菌系统重组蛋白表达
- 提供商：
普健生物（武汉）科技有限公司
Uniprot号：P24307
基因名：DNA replication helicase
蛋白名：DNA replication helicase
蛋白别名：
DNA replication helicase, EC:3.6.4.12, HELI, P143
服务内容：
| 服务项目 | 客户提供 | 服务内容 | 服务周期 | 交付内容 |
|---|---|---|---|---|
| 大肠杆菌系统 蛋白表达 | 基因序列 | 方案沟通 | 1天 | 方案报告 |
| 密码子优化和基因合成 | 5天 | 基因合成报告 | ||
| 表达纯化测试 | 3天 | 表达纯化测试报告 | ||
| 1L表达及纯化 | 3天 | 蛋白样品(3-5mg)及报告 | ||
| 放大发酵及纯化 | 7天 | 纯化的蛋白及报告 |
案例展示：
DNA replication helicase相关研究文献：
1. Nucleotide sequence of a gene essential for viral DNA replication in the baculovirus Autographa californica nuclear polyhedrosis virus. [1994581]
2. The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. [8030224]
3. Site-directed mutagenesis of the AcMNPV p143 gene: effects on baculovirus DNA replication. [9887325]
4. Baculovirus proteins IE-1, LEF-3, and P143 interact with DNA in vivo: a formaldehyde cross-linking study. [15518813]
5. Identification of domains in Autographa californica multiple nucleopolyhedrovirus late expression factor 3 required for nuclear transport of P143. [16103143]
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文献和实验2. The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. [8030224]
3. Site-directed mutagenesis of the AcMNPV p143 gene: effects on baculovirus DNA replication. [9887325]
4. Baculovirus proteins IE-1, LEF-3, and P143 interact with DNA in vivo: a formaldehyde cross-linking study. [15518813]
5. Identification of domains in Autographa californica multiple nucleopolyhedrovirus late expression factor 3 required for nuclear transport of P143. [16103143]
Generation of Recombinant Baculovirus DNA in E.coli Using a Baculovirus Shuttle Vector
of the Autographa californica nuclear polyhedrosis virus (AcNPV) are abundantly expressed during the late stages of infection. The recombinant proteins are usually soluble and functionally similar to their authentic counterparts (l –7 ). In the following sections
californica nuclear polyhedrosis virus (AcNPV), are most frequently used because they contain very active late gene promoters for proteins that are expressed late in the virus life cycle and are not essential for virus replication. Replacing a late gene
Introduction to the Molecular Biology of Baculoviruses
of one subgroup, the nuclear polyhedrosis viruses (NPVs). The ability to propagate baculoviruses in cell culture has also allowed extensive study of their molecular biology (10 ). The model virus in these studies is the Autographa californica NPV (AcNPV
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