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- 详细信息
- 文献和实验
- 技术资料
- 服务名称:
大肠杆菌系统重组蛋白表达
- 提供商:
普健生物(武汉)科技有限公司
Uniprot号:Q89592
基因名:Pre-hexon-linking protein VIII
蛋白名:Pre-hexon-linking protein VIII
蛋白别名:
12.1 kDa protein VIII, 7.6 kDa protein VIII, Hexon-linking protein-C, Hexon-linking protein-N, L4, Pre-hexon-linking protein VIII, Pre-protein VIII, Protein VIII-C, Protein VIII-N, pVIII
服务内容:
| 服务项目 | 客户提供 | 服务内容 | 服务周期 | 交付内容 |
|---|---|---|---|---|
| 大肠杆菌系统 蛋白表达 | 基因序列 | 方案沟通 | 1天 | 方案报告 |
| 密码子优化和基因合成 | 5天 | 基因合成报告 | ||
| 表达纯化测试 | 3天 | 表达纯化测试报告 | ||
| 1L表达及纯化 | 3天 | 蛋白样品(3-5mg)及报告 | ||
| 放大发酵及纯化 | 7天 | 纯化的蛋白及报告 |
案例展示:

Pre-hexon-linking protein VIII相关研究文献:
1. Complete nucleotide sequence from bovine adenovirus type 2. [CI-ABI7C5EQ9GL5E]
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
-galactopyranoside (X-gal; 25 mg/mL in DMF [dimethylformamide]) Agarose gel (1%, UltraPure; Invitrogen) Antibody, specific for the protein of interest Bead eluting buffer Bovine serum albumin (BSA; 0.5% in PBS; New England Biolabs) Cell
Protein‐Protein Interactions Identified by Pull‐Down Experiments and Mass Spectrometry
spectrometry, pull?downs can be considered as the protein?based equivalent of a yeast two?hybrid screen. To improve the isolation of specific binding partners, pull?down methods are described involving the use of cross?linking, large?scale tissue lysates
, NTPs, buffer, Mg++ ) Phenol/chloroform/isoamyl alcohol Protein A/G agarose beads (50%), pre-swollen and blocked (Santa Cruz, sc-2003) Proteinase K (20 mg/ml; store at -20°C) Random-primed DNA synthesis kit RIPA buffer
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