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- 详细信息
- 文献和实验
- 技术资料
- 服务名称:
大肠杆菌系统重组蛋白表达
- 提供商:
普健生物(武汉)科技有限公司
Uniprot号:P0C783
基因名:Suppressor of silencing 2b
蛋白名:Suppressor of silencing 2b
蛋白别名:
Protein 2b, Suppressor of silencing 2b
服务内容:
| 服务项目 | 客户提供 | 服务内容 | 服务周期 | 交付内容 |
|---|---|---|---|---|
| 大肠杆菌系统 蛋白表达 | 基因序列 | 方案沟通 | 1天 | 方案报告 |
| 密码子优化和基因合成 | 5天 | 基因合成报告 | ||
| 表达纯化测试 | 3天 | 表达纯化测试报告 | ||
| 1L表达及纯化 | 3天 | 蛋白样品(3-5mg)及报告 | ||
| 放大发酵及纯化 | 7天 | 纯化的蛋白及报告 |
案例展示:

Suppressor of silencing 2b相关研究文献:
1. Nucleotide sequence and evolutionary relationships of cucumber mosaic virus (CMV) strains: CMV RNA 2. [3404113]
2. Cucumber mosaic virus-encoded 2b suppressor inhibits Arabidopsis Argonaute1 cleavage activity to counter plant defense. [17158744]
3. Alanine scanning of cucumber mosaic virus (CMV) 2b protein identifies different positions for cell-to-cell movement and gene silencing suppressor activity. [25380036]
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文献和实验2. Cucumber mosaic virus-encoded 2b suppressor inhibits Arabidopsis Argonaute1 cleavage activity to counter plant defense. [17158744]
3. Alanine scanning of cucumber mosaic virus (CMV) 2b protein identifies different positions for cell-to-cell movement and gene silencing suppressor activity. [25380036]
Gel Mobility Shift Assays for RNA Binding Viral RNAi Suppressors
migrate slower through a non-denaturing polyacrylamide gel than the free nucleic acid fragments. Here, we provide a detailed protocol for the analysis of crucifer-infecting Tobacco mosaic tobamovirus (cr-TMV) silencing suppressor protein p122 RNA binding
and P o g u e, G. P . (2 0 0 2 ) Barley stripe mosaic virus-induced gene silencing in a monocot p lan t. Plant J. 3 0 , 3 15-327. 38. S h en , Q. -H ., S aijo , Y . , M au ch , S . , B isk u p , C ., B ieri, S . , K eller
sativa L .). J. Plant Physiol. 160, 931-9 4 3. 13. Meng, L ., Bregitzer, P ., Zhang, S. B. and Lemaux, P. G. (2003) Methylation of the exon/intron regionin the Ubil promoter complex correlates with transgene silencing in
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