Porcine reproductive and respi
ratory syndrome virus Nsp1-beta papain-like cysteine proteinase重组蛋白表达- ￥2000 - 8000
- 湖北省武汉市
- Porcine reproductive and respiratory syndrome virus Nsp1-beta papain-like cysteine proteinase重组蛋白表达
- 2026年04月08日
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- 详细信息
- 文献和实验
- 技术资料
- 服务名称：
大肠杆菌系统重组蛋白表达
- 提供商：
普健生物（武汉）科技有限公司
Uniprot号：P0DJY0
基因名：Nsp1-beta papain-like cysteine proteinase
蛋白名：Nsp1-beta papain-like cysteine proteinase
蛋白别名：
EC:3.4.22.-, Nsp1, Nsp1-alpha papain-like cysteine proteinase, Nsp1-beta papain-like cysteine proteinase, Nsp2TF, PCP1-alpha, PCP1-beta, Truncated polyprotein 1aTF
服务内容：
| 服务项目 | 客户提供 | 服务内容 | 服务周期 | 交付内容 |
|---|---|---|---|---|
| 大肠杆菌系统 蛋白表达 | 基因序列 | 方案沟通 | 1天 | 方案报告 |
| 密码子优化和基因合成 | 5天 | 基因合成报告 | ||
| 表达纯化测试 | 3天 | 表达纯化测试报告 | ||
| 1L表达及纯化 | 3天 | 蛋白样品(3-5mg)及报告 | ||
| 放大发酵及纯化 | 7天 | 纯化的蛋白及报告 |
案例展示：
Nsp1-beta papain-like cysteine proteinase相关研究文献：
1. Heterogeneity in Nsp2 of European-like porcine reproductive and respiratory syndrome viruses isolated in the United States. [15019241]
2. A full-length cDNA infectious clone of North American type 1 porcine reproductive and respiratory syndrome virus: expression of green fluorescent protein in the Nsp2 region. [16971421]
3. Efficient -2 frameshifting by mammalian ribosomes to synthesize an additional arterivirus protein. [23043113]
4. Transactivation of programmed ribosomal frameshifting by a viral protein. [24825891]
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文献和实验2. A full-length cDNA infectious clone of North American type 1 porcine reproductive and respiratory syndrome virus: expression of green fluorescent protein in the Nsp2 region. [16971421]
3. Efficient -2 frameshifting by mammalian ribosomes to synthesize an additional arterivirus protein. [23043113]
4. Transactivation of programmed ribosomal frameshifting by a viral protein. [24825891]
) virus and tentatively classified in the Arteriviridae family, which includes lactate dehydrogenase-elevating virus, equine arteritis virus, and simian hemorrhagic fever virus (2 ,5 ,6 ). The porcine reproductive and respiratory syndrome virus (PRRSV
PRRSV(Porcine Reproductive and Respiratory Syndrome Virus,猪繁殖与呼吸综合征病毒) was propagated in MARC-145 cells and purified by sucrose density gradient separation.Polyethylene glycol 8000(Sigma Chemical Co.,St.Louis,MO) at a final concentration of 8%(wt
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