- ￥2756
- OriGene
- 美国
- TA397723
- 2026年04月01日
- ELISA, IHC, WB
- Goat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Human IgG F(c) fragment
- 形态：
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
- 保存条件：
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
- 克隆性：
Polyclonal
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
TA397723
- 级别：
科研级
- 库存：
4周
- 供应商：
OriGene
- 宿主：
Goat
- 应用范围：
ELISA, IHC, WB
- 抗体英文名：
Human IgG F(c) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
- 抗体名：
Human IgG F(c) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
- 规格：
500 ug
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
in vitro. Fixation of chick brain whole mounts thus is typically done in 4% paraformaldehyde in PBS for 2-4 hours at 4O C. In areas where background alkaline phosphatase activity is a problem, increasing the time in 4% paraformaldehyde
Secondary antibody: Goat anti-mouse alkaline phosphatase conjugated - Biorad #170-6520. Substrate: ECF chemifluorescent substrate - Amersham #RPN5785. Mix substrate with accompanying buffer as per manufacturer’s
Western Blotting with Alkaline Phosphatase Conjugates
buffer (1% BSA, 10mM Tris pH 7.5, 100mM NaCl, 0.1% Tween 20). Incubate the blot for 30 minutes at 37°C, 1 hour at room temperature, or overnight at 4°C. INCUBATION WITH ALKALINE PHOSPHATASE CONJUGATED ANTIBODIES Dilute the AP
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