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- 详细信息
- 文献和实验
- 技术资料
- 形态:
PBS without preservatives.State: Low EndotoxinState: Liquid purified Ig fraction.
- 保存条件:
Store the antibody (in aliquots) at -20°C.Avoid repeated freezing and thawing.
- 克隆性:
Monoclonal
- 适应物种:
Mouse
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
AM08013LE-N
- 级别:
科研级
- 库存:
4周
- 供应商:
OriGene
- 宿主:
Rat
- 应用范围:
FC, IF
- 抗体英文名:
Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, Low Endotoxin
- 抗体名:
Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, Low Endotoxin
- 规格:
500 ug
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
me why this works!) before cloning out for variants. b) You can screen by ELISA or with mAb coupled RBC using mouse monoclonal antibodies against the Y3 derived Kappa 1a (RG11/15.5) and the specific DA Kappa 1b (G9/1.3), or the appropriate heavy chain (eg NORIG
Cloning and Expression of Single-Chain Fragments (scFv) from Mouse and Rat Hybridomas
information for VH and VL is generally amplified from hybridoma cells using the polymerase chain reaction (PCR) with antibody-specific primers A variety of primer sets for the amplification of mouse-variable domams has been developed that are particulary
Rapid Isotyping of Mouse Monoclonal Antibodies
for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance
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