pLV2-USP32-3×FLAG-Puro plasmid

pLV2-USP32-3×FLAG-Puro plasmid

PVT97184

pLV2-USP32-3×FLAG-Puro plasmid Information

Plasmid name: pLV2-USP32(human)-3×FLAG-Puro
Alias: NM_032582.4;NY-REN-60; USP10，Inducing agents should be added during extraction
Gene lenght: 4812bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): EPI400
Growth Temperature: 30°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: Low
Induction:

pLV2-USP32-3×FLAG-Puro plasmid Description

pLV2-USP32-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-USP32-3×FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatgggtgc caaggagtca cggatcggat tcctcagcta 2881 cgaggaggcg ctgaggagag ttacagatgt agagctaaaa cgactgaagg atgctttcaa 2941 gaggacctgt ggactctcat attacatggg ccagcactgc ttcatccggg aagtgcttgg 3001 ggatggagtg cctccaaagg ttgctgaggt gatttactgt tcttttggtg gaacatccaa 3061 agggctgcac ttcaataatt taatagttgg acttgtcctc cttacaagag gcaaagatga 3121 agagaaagca aaatacattt ttagtctttt ttcaagtgaa tctgggaact atgttatacg 3181 ggaagaaatg gaaagaatgc tccacgtggt ggatggtaaa gtcccagata cactcaggaa 3241 gtgtttctca gagggtgaaa aggtaaacta tgaaaagttt agaaattggc tttttctaaa 3301 caaagatgct tttactttct ctcgatggct tctatctgga ggtgtgtatg ttaccctcac 3361 tgatgatagt gatactccta ctttctacca aactctggct ggagtcacac atttggagga 3421 atcagacatc attgatcttg agaaacgcta ttggttattg aaggctcaat cccggactgg 3481 acgatttgat ttagagacat ttggcccatt ggtttcacca cctattcgtc catctctaag 3541 tgaaggtttg tttaatgctt ttgatgaaaa tcgtgacaat cacatagatt ttaaggagat 3601 atcctgtggg ttatcagcct gttgcagggg acccctggct gaaagacaaa aattttgctt 3661 caaggtattt gatgttgacc gtgatggagt tctctccagg gttgaactga gagacatggt 3721 ggttgcactt ttagaagtct ggaaggacaa ccgcactgat gatattcctg aattacatat 3781 ggatctctct gatattgtag aaggcatact gaatgcacat gacaccacaa agatgggtca 3841 tcttactctg gaagactatc agatctggag tgtgaaaaat gttcttgcca atgagttttt 3901 gaacctcctt ttccaggtgt gtcacatagt tctggggtta agaccagcta ctccggaaga 3961 agaaggacaa attattagag gatggttaga acgagagagc aggtatggtc tgcaagcagg 4021 acacaactgg tttatcatct ccatgcagtg gtggcaacag tggaaagaat atgtcaaata 4081 cgatgccaac cctgtggtaa ttgagccatc atctgttttg aatggaggaa aatactcatt 4141 tggaactgca gcccatccta tggagcaggt cgaagataga attggaagca gcctcagtta 4201 cgtgaatact acagaagaga aattttcaga caacatttct actgcatctg aagcctcaga 4261 aactgctggc agcggctttc tgtattctgc cacaccaggg gcagatgttt gctttgctcg 4321 acaacataac acttctgaca ataacaacca gtgtttgctg ggagccaatg ggaatatttt 4381 gttgcacctt aaccctcaga aaccaggggc tattgataat cagccattag taactcaaga 4441 accagtaaag gctacatcat taacactaga aggaggacga ttaaaacgaa ctccacagct 4501 gattcatgga agagactatg aaatggtccc agaacctgtg tggagagcac tttatcactg 4561 gtatggagca aacctggcct tacctagacc agttatcaag aacagcaaga cagacatccc 4621 agagctggaa ttatttcccc gctatcttct cttcctgaga cagcagcctg ccactcggac 4681 acagcagtct aacatctggg tgaatatggg aaatgtacct tctccgaatg cacctttaaa 4741 gcgggtatta gcctatacag gctgttttag tcgaatgcag accatcaagg aaattcacga 4801 atatctatct caaaggctgc gcattaaaga ggaagatatg cgcctgtggc tatacaacag 4861 tgagaactac cttactcttc tggatgatga ggatcataaa ttggaatatt tgaaaatcca 4921 ggatgaacaa cacctggtaa ttgaagttcg caacaaagat atgagttggc ctgaggagat 4981 gtcttttata gcaaatagta gtaaaataga tagacacaag gttcccacag aaaagggagc 5041 cacaggtcta agcaatctgg gaaacacatg cttcatgaac tcaagcatcc agtgtgttag 5101 taacacacag ccactgacac agtattttat ctcagggaga catctttatg aactcaacag 5161 gacaaatccc attggtatga aggggcatat ggctaaatgc tatggtgatt tagtgcagga 5221 actttggagt ggaactcaga agaatgttgc cccattaaag cttcggtgga ccatagcaaa 5281 atatgctccc aggtttaatg ggtttcagca acaggactcc caagaacttc tggcttttct 5341 cttggatggt cttcatgaag atcttaatcg agtccatgaa aagccatatg tggaactgaa 5401 ggacagtgat gggcgaccag actgggaagt agctgcagag gcctgggaca accatctaag 5461 aagaaataga tcaattgttg tggatttgtt ccatgggcag ctaagatctc aagtaaaatg 5521 caagacatgt gggcatataa gtgtccgatt tgaccctttc aattttttgt ctttgccact 5581 accaatggac agttatatgc acttagaaat aacagtgatt aagttagatg gtactacccc 5641 tgtacggtat ggactaagac tgaatatgga tgaaaagtac acaggtttaa aaaaacagct 5701 gagtgatctc tgtggactta attcagaaca aatccttcta gcagaagtac atggttccaa 5761 cataaagaac tttcctcagg acaaccaaaa agtacgactc tcagtgagtg gatttttgtg 5821 tgcatttgaa attcctgtcc ctgtgtctcc aatttcagct tctagtccaa cacagacaga 5881 tttctcctct tcgccatcta caaatgaaat gttcacccta actaccaatg gggacctacc 5941 ccgaccaata ttcatcccca atggaatgcc aaacactgtt gtgccatgtg gaactgagaa 6001 gaacttcaca aatggaatgg ttaatggtca catgccatct cttcctgaca gcccctttac 6061 aggttacatc attgcagtcc accgaaaaat gatgaggaca gaactgtatt tcctgtcatc 6121 tcagaagaat cgccccagcc tctttggaat gccattgatt gttccatgta ctgtgcatac 6181 ccggaagaaa gacctatatg atgcggtttg gattcaagta tcccggttag cgagcccact 6241 cccacctcag gaagctagta atcatgccca ggattgtgac gacagtatgg gctatcaata 6301 tccattcact ctacgagttg tgcagaaaga tgggaactcc tgtgcttggt gcccatggta 6361 tagattttgc agaggctgta aaattgattg tggggaagac agagctttca ttggaaatgc 6421 ctatatcgct gtggattggg atcccacagc ccttcacctt cgctatcaaa catcccagga 6481 aagggttgta gatgagcatg agagtgtgga gcagagtcgg cgagcgcaag ccgagcccat 6541 caacctggac agctgtctcc gtgctttcac cagtgaggaa gagctagggg aaaatgagat 6601 gtactactgt tccaagtgta agacccactg cttagcaaca aagaagctgg atctctggag 6661 gcttccaccc atcctgatta ttcaccttaa gcgatttcaa tttgtaaatg gtcggtggat 6721 aaaatcacag aaaattgtca aatttcctcg ggaaagtttt gatccaagtg cttttttggt 6781 accaagagac ccggctctct gccagcataa accactcaca ccccaggggg atgagctctc 6841 tgagcccagg attctggcaa gggaggtgaa gaaagtggat gcgcagagtt cggctgggga 6901 agaggacgtg ctcctgagca aaagcccatc ctcactcagc gctaacatca tcagcagccc 6961 gaaaggttct ccttcttcat caagaaaaag tggaaccagc tgtccctcca gcaaaaacag 7021 cagccctaat agcagcccac ggactttggg gaggagcaaa gggaggctcc ggctgcccca 7081 gattggcagc aaaaataaac tgtcaagtag taaagagaac ttggatgcca gcaaagaaaa 7141 tggggctggg cagatatgtg agctggctga cgccttgagt cgagggcatg tgctgggggg 7201 cagccaacca gagttggtca ctcctcagga ccatgaggta gctttggcca atggattcct 7261 ttatgagcat gaagcatgtg gcaatggcta cagcaatggt cagcttggaa accacagtga 7321 agaagacagc actgatgacc aaagagaaga tactcgtatt aagcctattt ataatctata 7381 tgcaatttcg tgccattcag gaattctggg tgggggccat tacgtcactt atgccaaaaa 7441 cccaaactgc aagtggtact gttacaatga cagcagctgt aaggaacttc acccggatga 7501 aattgacacc gactctgcct acattctttt ctatgagcag caggggatag actatgcaca 7561 atttctgcca aagactgatg gcaaaaagat ggcagacaca agcagtatgg atgaagactt 7621 tgagtctgat tacaaaaagt actgtgtgtt acagggatcc GACTACAAAG ACCATGACGG 7681 TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT GACAAGtagt ctagataatt 7741 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 7801 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 7861 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 7921 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 7981 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 8041 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg 8101 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag 8161 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc 8221 ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag 8281 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc 8341 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg 8401 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 8461 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg 8521 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 8581 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 8641 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 8701 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc 8761 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc 8821 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaccgcg tctggaacaa 8881 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 8941 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 9001 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 9061 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 9121 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 9181 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 9241 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc 9301 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 9361 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 9421 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc 9481 tgcagtcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat 9541 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct 9601 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa 9661 gaaaagaggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg 9721 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg 9781 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag 9841 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg 9901 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat 9961 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac 10021 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg 10081 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt 10141 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 10201 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 10261 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg 10321 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc 10381 ttgacattgc tagcgtttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat 10441 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 10501 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 10561 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 10621 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 10681 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 10741 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 10801 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 10861 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 10921 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 10981 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 11041 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 11101 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 11161 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 11221 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 11281 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 11341 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 11401 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 11461 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 11521 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 11581 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 11641 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 11701 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 11761 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 11821 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 11881 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 11941 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 12001 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 12061 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 12121 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 12181 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 12241 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 12301 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 12361 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 12421 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 12481 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 12541 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat 12601 cgggagatca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 12661 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 12721 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa 12781 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc 12841 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca 12901 aacttagtag tttttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.