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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
overnight or -70°C for 15 min. 13. Spin 12000g, 10 min, 4°C. Wash pellet with 70 EtOH and drain. Pellet should be a light pink or white. 14. Resuspend in 0.4 ml sterile gd H2 O in a microfuge tube. Precipitate with NaOAc and EtOH as in step 12.
Plant DNA methylation analysis by bisulfite genomic sequencing
(12000 X g); Discard supernatant, vacuum dry for 10 minutes at room temperature; Dissolve cut DNA in 10µl bidist water (0.25 or 2.5 µg/µl, respectively), store at -20°C; Bisulfite treatment The bisulfite solution
A quick RNA mini-prep for Neurospora mycelial cultures
pellet with 70% EtOH and drain. Pellet should be a light pink or white. 14. Resuspend in 0.4 ml sterile gd H2 O in a microfuge tube. Precipitate with NaOAc and EtOH as in step 12. 15. Spin 10-20 min in microfuge. Wash twice with 70% EtOH and dry
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