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- Thermo Fisher赛默飞热电
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- 2026年03月03日
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文献和实验Continuously automated synchronization of Plasmodium falciparum malaria parasites can be achieved by culturing in a custom temperature-cycling incubator (TCI) whose process controller is programmed to periodically change
Method: Preparation of Lymphoblastoid Cell Lines for Long Term Storage
and cells to cool to the start temperature of + 4 degrees C. Place the selector switch to the freeze ampule position. The controller will automatically cycle through the freezing program until the end temperature is reached
Protocols for ET recombination
temperature is important, usually 60℃ - 62℃ is optimal. Purify the PCR products by Qiagen column and elute with 2x 50 μl dH2O. Add 10x Dpn 1 buffer and 2 μl Dpn 1 (NEB) and digest (to eliminate template DNA) for 1 hour. Extract with Phenol:CHCl
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