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文献和实验V294.PART II,Chapter 4 细胞迁移实验之Dunn趋化迁移培养皿法及时序显微镜分析法
with distilled water and stored in100% ethanol until use. Before use the ethanol is removed from each cover slipby sweeping it through a naked flame using tweezers until all the ethanol hasevaporated. This also serves to sterilize the cover slip. 3. Dunn
Procedures for In Vitro Production of Bovine Embryos
in front of the heater) Heater Scissors (wipe with ethanol)Semen straw plunger (wipe with ethanol)Light microscope Plastic sterile Pasteur pipets Pipettor (25 ml) Pipet tips Instrument to pick-up oocytes Slide warmer (set at 38.5°C)Procedure1. Place X-plate
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
tip) alternatively: Finger controlled pipette (Manostat tubing, yellow tip, scalpel blade); 9'' Pasteur pipettes; 70% Ethanol; Alcohol burner or Bunsen burner; Sterile plastic Petri dishes (100 x 15 mm); Sterile
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