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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
连桥生物
- 现货状态:
现货
- 保修期:
2年
- 规格:
台
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文献和实验of transgenic wheat with an antisense construct directed against the thioredoxin. Z h i W uS h en g L i Y u F en Z i S h en g W u X u e X u e B a o 33, 18-24. 46. M a rtin , J. M . , M eyer9 F. D . , S m id a n sky,
Large Scale Tubulin Preparation
for resuspending the chunky pellets. Check protein concentration by Bradford using BSA as a standard. If >20 mg/ml (which is unlikely), dilute to 20 mg/ml. 6. Spin the depolymerization mixture 30' at 35K in 2 Type 45 rotors at 4¡C. At the end of the spin set
Large Scale Tubulin Preparation
(cold; need to warm up one after 1st cold use) 1 Type 50.2Ti rotor (cold) cold = 4¡C (put overnight in cold room) warm = 37¡C (put the rotors overnight in a large bacterial shaker set to 37¡C) VI. Protocol
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