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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见说明书
- 保质期:
见说明书
- 英文名:
见说明
- 库存:
100
- 供应商:
连桥生物
- CAS号:
见说明书
- 规格:
250mg/瓶
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文献和实验In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
In Vitro Transcription Assay (Run-off Assay) using Permeabil
. Centrifuge at maximum speed (13,000 rpm) in a microcentrifuge for 10 min at 4℃ to pellet the RNA. 19. Allow the pellet to air dry. Avoid over-drying the pellet. 20. Resuspend the RNA pellet in DEPC-treated TE or DEPC-treated ddH2 O (see Hint #3). D
one-step reaction (25).Each extension reaction contained 500-750ng of Biomek isolated single-stranded DNA, reaction buffer, nucleotide extension mix, oligonucleotide primer (typically M13 (-40) universal sequencing primer, see Appendix D), either [a-32-P
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