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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
250mg
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文献和实验Biotin labeling and hybridization of probe to chromosomes
sheared salmon sperm DNA (10 mg/ml) 2 µl probe DNA 0.5-2 µl Deionized or distilled water
Preparation of Genomic DNA from Bacteria- using Phase Lock GelTM
Materials: see Solutions for Recipes TE buffer 10% (w/v) sodium dodecyl sulfate (SDS ) 20 mg/ml proteinase K
Yeast Nuclear Extract (Small Scale)
. Dialyze nuclear extract vs Buffer C+ 75 mM ammonium sulfate at 4o.Dialyze vs 500ml buffer with 3 changes of buffer over 4.5 hours total. Aliquot extract and store at -70 degrees.Extracts should be 25-50 mg/ml in protein. BioRad Protein Assay of extracts
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