重组葡激酶(rSAK)
Recombinant Staphylokinase
(rSAK)
PrimeGene Technical Data Sheet
Catalog Number:
451-02
Source:
Escherichia coli.
Molecular Weight:
Approximately 15.6 kDa, a single non-glycosylated polypeptide chain containing 136 amino acids.
Quantity:
20μg/100μg/1000μg
AA Sequence:
SSSFDKGKYK KGDDASYFEP TGPYLMVNVT GVDGKRNELL SPRYVEFPIK
PGTTLTKEKI EYYVEWALDA TAYKEFRVVE LDPSAKIEVT YYDKNKKKEE
TKSFPITEKG FVVPDLSEHI KNPGFNLITK VVIEKK
Purity:
>95% by SDS-PAGE and HPLC analyses.
Biological Activity:
Fully biologically active when compared to standard. The specific activity determined by fibrining
lysis in agarose plate is 5.0 × 10
4
IU/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:
Lyophilized from a 0.2
μ
m filtered concentrated solution in PBS, pH 7.4.
Endotoxin:
Less than 1EU/
μ
g of rSAK as determined by LAL method.
Reconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a
concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
stored at <
-20
°
C. Further dilutions should be made in appropriate buffered solutions.
Storage:
This lyophilized preparation is stable at 2-8
°
C, but should be kept at -20
°
C for long term storage,
preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8
°
C. For
maximal stability, apportion the reconstituted preparation into working aliquots and store at -20
°
C to
-70
°
C.
Avoid repeated freeze/thaw cycles
.
Usage:
This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further
evaluation purposes.
NOT FOR HUMAN USE
.
Staphylokinase
Staphylokinase (SAK), a 16kDa profibrinolytic protein from the Staphylococcus aureus, has been demonstrated to induce highly
fibrin-specific thrombolysis in both human plasma and in limited clinical trials. Recent studies on the thrombolytic potential
of
recombinant SAK in achieving early perfusion in myocardial infarction and in the dissolution of platelet-rich clot have clearly
established its immense utility in clinical medicine as a thrombolytic agent and suggested that it can be developed as a potent
clot-dissolving agent.