Anti-LMA1L

原创：western protocol

1.5M Tris(PH 8.8) 100ml 18.171g (at4°C) 10% SDS 100 ml 10g (at room temperature) 10% ammonium persulfate 1ml 0.1g(replace every week store at at4°C) TEMED 8 room temprature Antibodies Primary antibody: 5A11(anti-mouse IgG) 500x-1000x Secondary

Dynabeads® CD3CD28 CTS

using Dynabeads® CD3⁄CD28 CTS™. Following separation, the CD3 T cells are cultured in the presence of the beads. By combining anti-CD3 and anti-CD28 antibodies on Dynabeads®, the beads will provide both the primary and co-stimulatory signals

Purification of mAb (IgG)

needles. Binding Buffer (BioRad): make 1L. 314g/L ddH2 O and filter through 0.22 um, filter. pH=9.0 Elution buffer (BioRad): make 500 ml. 11g/500 ml ddH2 O and filter as before. pH=3.0 Regeneration buffer (BioRad): BioRad Affi-gel