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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
苏州瑞诺德生物科技有限公司
- 规格:
100 ug
抗体
经严格验证并文献引用的Invitrogen品牌一抗、二抗,可满足多种实验应用,如WB、IHC/ICC/IF、ELISA等。
使用Invitrogen抗体品牌页面顶部检索框,从10万多Invitrogen抗体库中筛选合适的抗体。
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文献和实验Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
Rapid Isotyping of Mouse Monoclonal Antibodies
for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance
Immunofluorescent Staining of Mouse and Rat Leukocytes
secondary reagent (e.g., fluorochrome-conjugated avidin, streptavidin, anti-Ig allotype, anti-Ig isotype, polyclonal anti-Ig). For example, dilute antibody to ~1 µg per 100 µl in wash buffer and add this to each well containing the loosened cell pellet
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