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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
LightCycler 8-Tube Strips (white)
- 供应商：
上海汇谱仪器有限公司
- CAS号：
459115-SR
- 规格：
10 x 12 根8联管 (白色，带盖)
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文献和实验Transformation of Electrocompetent E. coli with Blue/White selection
to a bacteria tube. Repeat for additional DNA samples and control DNA. Rotate at 37ºC x30 min. to allow the bacteria to recover. Meanwhile plate LB/Amp plates with IPTG + X-gal if blue/white selection of colonies will be performed
).Transfer serum/white blood cells to 50 ml tube, underlay 12 ml RT Histopaque 1077.Spin 2500 rpm/30min at RT. To take mononuclear cells:Take off serum above band. (See attached figure, in preparation)Take mononuclear band + some Ficoll into two 15ml tubes
the unhomogenized material at the bottom of the centrifuge tube. The protein concentration in the collected fraction was measured according to the Bradford dye-binding procedure [9]. The original protein extract was diluted in rehydration solution containing 8 M
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